DNA-RNA杂交体抗体 [S9.6]

¥3,780.00

规格：100ug
克隆：鼠单抗
克隆号：S9.6

SKU：ENH001 分类：抗体品牌： Kerafast

广州泛思生物竭诚为您服务

产品详情

DNA-RNA杂交体抗体ENH001/ENH002

Anti-DNA-RNA Hybrid [S9.6] Antibody

在真核细胞中，DNA–RNA杂合体是自然存在的；在转录活性较高的位点，如转录起始、抑制及延伸阶段，这类杂合体的水平会升高。由于RNA–DNA杂合体会影响基因组的不稳定性，S9.6抗体是一种有效的试剂，可用于研究R环以及由这些杂合体在DNA复制或其他细胞过程中形成的损伤所导致的后果。此外，S9.6抗体在微阵列研究中能够有效识别RNA-DNA杂交。

该小鼠单克隆抗体是针对ΦX174噬菌体来源的合成DNA-RNA抗原制备的，并可识别不同长度的RNA-DNA杂合体。可特异性结合≥6 bp的RNA-DNA杂交双链结构（经SPR验证解离常数Kd=3.2 nM）

R-loop检测利器

可高效检测DNA-RNA杂交体与游离RNA链形成的R-loop结构（Useful in the detection of R-loops）

高特异性结合

对DNA-RNA杂交体具有显著亲和力（经SPR验证Kd=3.2 nM）（High specificity and affinity for DNA-RNA hybrids）

严格交叉反应控制

•不与单链DNA（ssDNA）或双链DNA（dsDNA）发生交叉反应•对富含AU的双链RNA（dsRNA）有微弱交叉反应（灵敏度低约5倍）

广谱长度识别

可稳定结合8、10、15及23个碱基对长度的杂交体（经ELISA验证）（High affinity binding shown for hybrids of 8, 10, 15, and 23 base pairs in length）

Anti-DNA-RNA Hybrid [S9.6] Antibody

Product Type:	Antibody
Name:	Anti-DNA-RNA Hybrid [S9.6]
Antigen:	S9.6 ΦX174 bacteriophage-derived synthetic DNA–RNA antigen
Isotype:	Mouse IgG2a
Clonality:	Monoclonal
Fusion Tag(s):	Mouse Fab version contains His-tag
Clone Name:	S9.6
Reactivity:	High specificity and affinity for DNA/RNA hybrids and other A-form nucleic acid hybrids
Immunogen:	ΦX174 bacteriophage-derived synthetic DNA/RNA
Species Immunized:	BALB/c mouse
Paratope:	DYYGSRWFDY (proposed)
Purification Method:	Protein A/G
Buffer:	ENHOO1: PBS, 0.05% (w/v) Sodium Azide Ab01137- : PBS with 0.02% Proclin 300
Tested Applications:	Dot Blot Analysis: 0.2 µg/mL. Affinity Binding Assay: Clone S9.6 bound the DNA-RNA heteropolymer and poly(I)-poly(dC) equally, but 100-fold higher levels of poly(A)-poly(dT) were required to achieve a similar degree of binding. Single-stranded DNA, double-stranded DNA and RNA, and ribosomal RNA were not bound by clone S9.6 (Boguslawski, S.J., et al. (1986). J. Immunol Methods. 89(1):123-130). Chromatin Immunoprecipitation (ChIP) Analysis: A representative lot detected increased DNA RNA hybrids at four actively transcribed genes upon shRNA-mediated knockdown of BRCA1 or BRCA2, but not PCID2 or RAD51 in HeLa cells (Bhatia, V., et al. (2014). Nature. 511(7509):362-365). Chromatin Immunoprecipitation (ChIP) Analysis: A representative lot detected R-loops formed over beta-actin gene using HeLa chromatin preparation. RNase H treatment of the chromatin preparation prevented clone S9.6 from immunoprecipitating target chromatin fragments (Skourti-Stathaki, K., et al. (2011). Mol. Cell. 42(6):794-805). Chromatin Immunoprecipitation-sequencing (ChIP-seq) Analysis: A representative lot detected genome-wide distribution of DNA-RNA hybrids in budding yeast by ChIP-seq analysis (El Hage, A., et al. (2014). PLoS Genet. 10(10):e1004716). Immunocytochemistry Analysis: Representative lots immunolocalized nuclear R loops by fluorescent immunocytochemistry staining of methanol-fixed H1 human embryonic stem cells (hESCs) and formaldehyde-fixed HeLa cells (Bhatia, V., et al. (2014). Nature. 511(7509):362-365; Ginno, P.A., et al. (2012). Mol. Cell. 45(6):814-825). Immunoprecipitation Analysis: A representative lot immunoprecipitated in vitro transcribed R-loop substrate (DNA-RNA hybrid), but not doouble-stranded DNA (dsDNA) (Ginno, P.A., et al. (2012). Mol. Cell. 45(6):814-825). See also: S9.6 Publications by Application
Storage:	-20C (avoid repeated freeze-thaw cycles)
Shipped:	Cold packs